E. coli-induced inflammation

Escherichia coli (E. coli) are part of the normal intestinal flora but are also the most common cause of both lower and upper urinary tract infections. Upper urinary tract infection may spread to the bloodstream and cause sepsis and E. coli is the bacterium most commonly isolated in blood cultures. Knowledge on the involvement of inflammasomes in E. coli infections is sparse and is suggesting TLR (Toll-like receptors) as master regulator of inflammasome activation. 

This study investigates the host response to E. coli infections focusing on the role of TLR and NLR (Nod-like receptors) in inflammasome activation and regulation. We use well-characterised uropathogenic E. coli strains (e.g. CFT073) in our search to understand which virulence factors that induce what mechanisms. Clinical isolates, from patients with cystitis, pyelonephritis and urosepsis, collected at Karolinska University Hospital and characterized regarding virulence factors, will also be employed.